Comic created by a fellow vet, Pepe Gonzalez, happily discharged and a few years old.
Friday, November 30, 2007
Myoelectric Arm Purchase
The Ball Felisin
Comic created by a fellow vet, Pepe Gonzalez, happily discharged and a few years old.
Comic created by a fellow vet, Pepe Gonzalez, happily discharged and a few years old.
Thursday, November 22, 2007
Blog Chapstick Favors
The History (I)
Behind this little word, incomprehensible to many, it simply hides the data collection takes the vet to start an animal's medical history.
is a process as important as the clinical examination or laboratory tests, which aims to summarize information on all spatial and temporal circumstances surrounding the pet.
And it is a phase of the clinical process in which the owner is key.
this post and then claim to be a small guide tips useful for conscientious owners with their participation in the care of your pet. Although most of them relate to dogs and cats, can be understood for any sick pet. A veterinarian will always appreciate the full details and as accurate as possible about the animal being treated.
WHO GOES TO ANIMAL?
a decision may seem banal, the person who comes to the clinic is the link between the veterinarian and the animal, and unfortunately, these do not speak. Always be the closest person, which spend more time with him and therefore we know better. In the event that it is an older child to take care of it, it should I go to the clinic himself, yes, accompanied by an adult.
This is so for two reasons: First, the animal will be more confident and calm if you trust the person who accompanies and subject. Secondly, not least because it is this person who knows the details better and is able to remember more. Although the veterinarian
wondering, should remember before going to the clinic, the following information:
SPECIES
obviously not necessary in the case of dogs and cats, but especially with reptiles and rare birds, and help meet the genus and species of animal, and there is not always literature specialized, and thus be more accurate treatment. AGE
years
know who has a dog, or months of a puppy, even if approximate, if an animal is adopted as an adult, is a valuable information for clinicians, since there are pathologies that are largely or exclusively to age. LIFESTYLE
Do you live in an apartment or a house with garden? Where do you sleep? How many times a day out? Have you recently changed your home? Have there been any drastic changes in the family you live with the animal? (Birth of a baby, loss of a person who looked after him ...)¿ Travel with your pet? "An alien? "Coexists with other animals of the same or different species? Do you brush and bathe regularly? Do you have toys? What kind? Do you use? Do you exercise regularly? How hard? How tolerate animal? Have you always been this way? Have you ever run away? In the case of parrots What room of the house is placed a cage? What is walking on it for her? ...
These and other questions about lifestyle can be carried out by the vet, it would be desirable to have clear answers. SEXUAL STATUS
Is neutered / a? How long? If not, in the case of females had When the last heat? (Nope, very important information to know when the cycle in which the animal is, or ferrets predict the severity of certain diseases and the urgency of treatment. Note the date provided) Did you have litters? How many times? How many puppies did you have? Did you have any problems in pregnancy or childbirth?
FOOD.
not just have to know what you eat (in the case of dogs, I know the kind of lets us know the composition of the protein, important in the treatment of food allergies) but how much. Although it is very useful as they used to mark the daily amount as this can work with accurate and not just approximate.
It is also important to mention the occasional food, the kind of goodies that are given as prizes, and generally anything that the animal has been swallowed (for example, a cat that has caught a bird has eaten part of it) . Appetite changes observed are also part of this section: Animals that eat more or less normal, they eat abnormal substances such as sand, or their own feces, or grass. (No. It is not normal for a dog "purged" . is indicative of disease) is also of course important to mention if we learn that the pet has been eating a foreign object, such as broken toys, socks, plates or bone (Very dangerous! Avoid them at all costs) Note
drink deserves. Is a key symptom and vital called polydipsia (animals who drink more than normal) in many common diseases in the clinic. If you know the information about how much an animal about 24 hours, you can detect alterations in the pattern, and also in the case of some birds and reptiles administer drugs in the drinking water with some certainty as to the dose.
Behind this little word, incomprehensible to many, it simply hides the data collection takes the vet to start an animal's medical history.
is a process as important as the clinical examination or laboratory tests, which aims to summarize information on all spatial and temporal circumstances surrounding the pet.
And it is a phase of the clinical process in which the owner is key.
this post and then claim to be a small guide tips useful for conscientious owners with their participation in the care of your pet. Although most of them relate to dogs and cats, can be understood for any sick pet. A veterinarian will always appreciate the full details and as accurate as possible about the animal being treated.
WHO GOES TO ANIMAL?
a decision may seem banal, the person who comes to the clinic is the link between the veterinarian and the animal, and unfortunately, these do not speak. Always be the closest person, which spend more time with him and therefore we know better. In the event that it is an older child to take care of it, it should I go to the clinic himself, yes, accompanied by an adult.
This is so for two reasons: First, the animal will be more confident and calm if you trust the person who accompanies and subject. Secondly, not least because it is this person who knows the details better and is able to remember more. Although the veterinarian
wondering, should remember before going to the clinic, the following information:
SPECIES
obviously not necessary in the case of dogs and cats, but especially with reptiles and rare birds, and help meet the genus and species of animal, and there is not always literature specialized, and thus be more accurate treatment. AGE
years
know who has a dog, or months of a puppy, even if approximate, if an animal is adopted as an adult, is a valuable information for clinicians, since there are pathologies that are largely or exclusively to age. LIFESTYLE
Do you live in an apartment or a house with garden? Where do you sleep? How many times a day out? Have you recently changed your home? Have there been any drastic changes in the family you live with the animal? (Birth of a baby, loss of a person who looked after him ...)¿ Travel with your pet? "An alien? "Coexists with other animals of the same or different species? Do you brush and bathe regularly? Do you have toys? What kind? Do you use? Do you exercise regularly? How hard? How tolerate animal? Have you always been this way? Have you ever run away? In the case of parrots What room of the house is placed a cage? What is walking on it for her? ...
These and other questions about lifestyle can be carried out by the vet, it would be desirable to have clear answers. SEXUAL STATUS
Is neutered / a? How long? If not, in the case of females had When the last heat? (Nope, very important information to know when the cycle in which the animal is, or ferrets predict the severity of certain diseases and the urgency of treatment. Note the date provided) Did you have litters? How many times? How many puppies did you have? Did you have any problems in pregnancy or childbirth?
FOOD.
not just have to know what you eat (in the case of dogs, I know the kind of lets us know the composition of the protein, important in the treatment of food allergies) but how much. Although it is very useful as they used to mark the daily amount as this can work with accurate and not just approximate.
It is also important to mention the occasional food, the kind of goodies that are given as prizes, and generally anything that the animal has been swallowed (for example, a cat that has caught a bird has eaten part of it) . Appetite changes observed are also part of this section: Animals that eat more or less normal, they eat abnormal substances such as sand, or their own feces, or grass. (No. It is not normal for a dog "purged" . is indicative of disease) is also of course important to mention if we learn that the pet has been eating a foreign object, such as broken toys, socks, plates or bone (Very dangerous! Avoid them at all costs) Note
drink deserves. Is a key symptom and vital called polydipsia (animals who drink more than normal) in many common diseases in the clinic. If you know the information about how much an animal about 24 hours, you can detect alterations in the pattern, and also in the case of some birds and reptiles administer drugs in the drinking water with some certainty as to the dose.
How To Make Biscuits From Krusteaz Pancake Mix
Make-me-me Kittlers
" will send a handmade gift to the first three people, animals or things that answer to this post, but they must commit to do the same in yours ... How long? in 365 days! And what to send? Anything worth handmade scarves, crochet, cross stitch, decoupage, jewelry, baskets, cards, clothes, whatever, but done by hand and me. That if the three individuals selected must commit to do the same on your blog! "
Go ejemplitos ... if anyone knows what the decoupage (not worth googling, cheaters) to tell me, please ...
meme I came DaNi
" will send a handmade gift to the first three people, animals or things that answer to this post, but they must commit to do the same in yours ... How long? in 365 days! And what to send? Anything worth handmade scarves, crochet, cross stitch, decoupage, jewelry, baskets, cards, clothes, whatever, but done by hand and me. That if the three individuals selected must commit to do the same on your blog! "
Go ejemplitos ... if anyone knows what the decoupage (not worth googling, cheaters) to tell me, please ...
meme I came DaNi
Wednesday, November 21, 2007
Wiring Honeywell Rth310c
Internet, paradise of freaks.
also of freaks-pet owners. Today
on their screens ... Cats that look like Hitler!
http://www.catsthatlooklikehitler.com
* and remember: The cat is not to blame.
Tuesday, November 20, 2007
How Much Does John Cryer Make
all have an idea about clear about epilepsy in humans, either because they know someone with this problem either because it has been discussed extensively in the film ("A spoon so they do not bite your tongue!", etc) but ... what about pets? Can you have this problem? What are the symptoms?
believe it or not, is a relatively common problem in small animal clinic. Between 0.5 and 5% of dogs can have it, and these are not all those with seizures. So first we will see the difference between one thing and another, since all the animals suffering from epilepsy syndromes manifest as convulsive but not all seizures are caused by epilepsy.
A seizure is a disorder of brain function (an abnormal electrical discharge in the action potentials of neurons) which by definition is paroxysmal (appearing suddenly) transient (a brain disorder is not continuous, as could be for example tremors in Parkinson's disease), stereotyped (with a fixed motion pattern, repeated over and over again) and involuntary. The causes are complex but can be summarized in an alteration of neurotransmitters (chemicals that neurons use to communicate with each other) and an imbalance of these with a loss of inhibitory neurotransmitters for the drivers.
seizures alterations at three levels:
-in level of consciousness
-motor-in the autonomic nervous system.
Depending on the magnitude of these problems, and their different involvement in the process, we can observe various types of seizures, from alternating phases of contraction and relaxation throughout the body of the animal, to seizures without muscle movements (which are confused with syncope or fainting), through partial seizures (the animal is moved only a foot) or so-called complex partial alteration in the temporal lobe produces sensory disturbances (sounds strange visions) that If a dog can see how disturbed behavior.
Some poisonings and metabolic problems (eg hypoglycemia in an animal escaped, he has not eaten in several days, or do not eat any other cause) may cause seizures without epilepsy, and so it is easy to confuse this type problems with syncope, or if the animal has a very painful abdominal or column, with the manifestation of this pain. For all this, it is very important that the diagnosis is performed by a veterinarian, since the seizure is a symptom that is warning us that there may be a major problem that must be addressed.
Epilepsy is a syndrome with seizure primary cause (the problem) within the cerebral cortex, and recurrent course, which means that crises occur periodically and is not a single isolated episode.
We distinguish between two types of epilepsy:
-Idiopathic epilepsy or true: Very rare in the cat. When we find no cause.
-secondary or symptomatic epilepsy, caused by congenital malformations (in puppies) head trauma (hit dogs, cats fallen from upper floors ...) infections, tumors, strokes ...
In the first case, the symptoms can be recognized as owners are abnormal animal behavior prior to the crisis, after which the animal collapses (usually sideways) and then a general rigidity of all muscles, which are alternating phases of contraction and relaxation. Furthermore, excessive drooling is a common, or that the animal urinated on himself. The crisis last 1 to 3 minutes, and recovery will be longer depending on the intensity and duration of the crisis.
In the second case, we can see different types of seizures to a greater or lesser degree, depending on the cause and the area of \u200b\u200bthe nervous system is affected.
Treatment aims to reduce the frequency, duration and severity of the crisis, not its demise and unfortunately this is not possible. Sometimes is a lifelong treatment, but depending on the clinical response of the animal, it is sometimes possible to reduce the dose and be phased out. In any case it is very important that the animal is controlled by your veterinarian, and timely compliance with the prescribed controls.
In the case of secondary epilepsy, does not help control seizures if they are not attacking the root cause (tumor, infection ...) as these are only a warning that something is wrong underneath.
Treatment is urgent if observed :
-2 or more crises in a month.
-Crisis in runs (several in one day)
-crisis threatening the life of the animal, even if they are isolated
-Crisis over 5 minutes.
Saturday, September 29, 2007
How To Know If A Steep Tech Is
Solidarity Epilepsy Seizures vs
In my school, people are very supportive.
Seriously, it is. As someone mentioned any campaign or movement against animal cruelty, fair trade or third world, usually have a great reception and acceptance.
Now, that, from what I see, is only out of doors, because with one's peers, which are those that at some point they'll pull through, and who can do a favor at a given time is something else ...
will say that these notes are his work, she seems worthy of and has the right to charge for them.
The law, Rocio, you have it, of course. Now, I do not see you ever been delegated, or asking someone for a class that you could not go, right? Or at least not without wearing a pair of eurillos in his pocket, and a contract of assignment of rights, in order to sell you later.
What career awaits you, beautiful. I pity you. PD
already put the note to give an idea of \u200b\u200bthe "quality", I think a joke .... 
In my school, people are very supportive.
Seriously, it is. As someone mentioned any campaign or movement against animal cruelty, fair trade or third world, usually have a great reception and acceptance.
Now, that, from what I see, is only out of doors, because with one's peers, which are those that at some point they'll pull through, and who can do a favor at a given time is something else ...
will say that these notes are his work, she seems worthy of and has the right to charge for them.
The law, Rocio, you have it, of course. Now, I do not see you ever been delegated, or asking someone for a class that you could not go, right? Or at least not without wearing a pair of eurillos in his pocket, and a contract of assignment of rights, in order to sell you later.
What career awaits you, beautiful. I pity you. PD
already put the note to give an idea of \u200b\u200bthe "quality", I think a joke ....
Wednesday, September 12, 2007
What Kind Of Hair Does Keri Hilson Wear
Malo vet!! Signal
" To train your dog, my recommendation is this: When does something wrong, cut a piece of his tail and give eating. Then do not give anything more than the rest of the day "
Courtesy of Miss Selene, who found him diving around ...
The title of this post is a nod to some anonymous commenter who threatened to tell everyone that I was a bad vet "... What else would I, my good man, to be as veterinary, bad or good ...
" To train your dog, my recommendation is this: When does something wrong, cut a piece of his tail and give eating. Then do not give anything more than the rest of the day "
Courtesy of Miss Selene, who found him diving around ...
The title of this post is a nod to some anonymous commenter who threatened to tell everyone that I was a bad vet "... What else would I, my good man, to be as veterinary, bad or good ...
Monday, September 10, 2007
Cockroach Poop Images
teaching to avoid heat stroke
Clear, simple and straightforward.
Eye but this summer was not the hottest, with encerrrado leave an animal in a car in the sun. 
Clear, simple and straightforward.
Eye but this summer was not the hottest, with encerrrado leave an animal in a car in the sun.
Saturday, September 8, 2007
Hans Steamer Cleaning Pads
not the "Gambit" (Feed Myths I)
One of the things that frustrates us greatly to be studying veterinary medicine (or having completed), is that everyone remembers you when your pets are sick (well, it's a step, I give advice whenever I can, but imposition of and without instrumental hands can not even diagnose or cure, and most of the time my best advice is to be taken to a clinic), and yet almost no one does for advice when purchasing or donating a new pet.
And it'll be quite useful most of the time, and that can be corrected "baseline" before the animal and owner to acquire bad habits of feeding and management, and often allows you to save a part of the financial outlay, which can be reinvested in other items that really are necessary.
My idea is to make a small series of post on the most frequent errors with the most common pet, beginning today by the Florida turtle or red-eared slider (Trachemys scripta elegans )
These turtles (it would more correct to say freshwater), had their boom in popularity as pets in the mid-late 80 (I had myself a little). I do not usually recommend them to anyone who asks me, as this adorable turtle:
going to turn into the other within 5-7 years, and may even grow a little more.
And what about the turtle in an aquarium crowded too small for her, kinda (because not deceived; of sea water smells really bad), and that once the initial excitement has just cornered, with no one to change the times you should? Well, the best was dying (yes, you read that right), and at worst ended up being "liberated" in the river / lake / stream near you.
Being voracious species, ecosystems accustomed to more hostile to a reptile as those encountered on the peninsula, easily displace native freshwater, Amphibians consume sunsets, etc, causing real harm to the precarious balance of the basin fauna.
And it's not just isolated individuals: There is evidence of established populations and reproduction in the wild, making it difficult to stop this disaster ... In
GREFA(Group for the recovery of native wildlife and its habitat) is engaged in collecting specimens that people can no longer keep in his house, to prevent uncontrolled loose:
RELEASE MUST NOT BE A COMPANION ANIMAL IN NATURE.
View the magnitude of the problem, we decided to create obstacles to trade in the species, to avoid as far it may be repeated loose. The savvy importers soon solved this little work, going to sell instead of prohibited species, the "yellow-eared turtle ( Chrisemys picta), almost indistinguishable from the previous to the general public.
The problem has been reduced in part through environmental education "guerrilla" (in clinics, such as information from friends, from subjects in the faculties ...), but has not conducted any campaign at government and in many cases continue the uncontrolled loose.
But anyway, once the animal is in our home, and we have made clear that in no event will we release it in nature, our responsibility is to be in the best possible conditions. Although they are very resistant bugs is not a question of testing this resistance, compromising the welfare of the pet.
And we will speak first of accommodation:
In most cases (much more if the turtle is going to be for a child), the animal usually accompanied by a device similar to the picture:
Palmerita
With its plastic, its fancy ramps .... some even have a swimming pool in the island. And without going into the hassle involved in changing the water every two days or so that the turtle does not have room to swim properly What to do when the bug measures about 20 cm long by 13 wide?
The most suitable for all aquatic reptiles (including this one) is a aquaterrariums, with a portion of water for at least the depth of the width of the turtle, and a ground, with easy access to allow the sunlight, and a substrate which can be partially buried, and wear down their nails.
portion A water filter is a very good investment, and significantly reducing the attention we pay to the animal, which culminate in the end is cleaner. Are quite resistant to our temperatures, but ideally should also have a water heater to prevent dozing in winter.
All reptiles (especially daytime) need to sunbathe daily or have a special fluorescent reptiles to include in its spectrum UVA and UVB rays necessary for the proper synthesis of vitamin D and calcium absorption. To maintain its effectiveness this tube should be changed every 6 months.
accommodation is in the initial (I recommend that it consider the action to take the animal to its growth) we must invest more attention and money. All the money we save on food.
From here I start a blog-media campaign that it takes time to develop in veterinary clinics served by responsible:
JUST ONCE WITH THE THYMUS OF THE GAMBIT.
The shrimps (Gammarus) are small freeze-dried shrimp that are sold to feed red-eared turtles (used to come in the pack next to the turtle of the Palmerita). 90% of the product is the chitinous exoskeleton (if you do not believe me, crush one between the fingers) a protein of very low biological value, lacking essential amino acids and is by no means a complete food for a reptile in the middle natural feeds fish, eggs, tadpoles, insect larvae, algae ...
But besides all this, we would be cheaper (at equal weight) to feed our turtle with caviar! (And would be more nutritious)
For your own physiology, turtles are adapted to a heterogeneous diet. Healthier for the turtle and will feed it with our pocket:
"I think the kitten. Diet is an excellent base for a lot of animals (true Pip?). At first we chop the pellets to fit into the mouth of the turtle. Then not be necessary. There are animals that consume even crisp, nothing but toss, and other expected to have softened.
-Chicken Liver. We offer it cut into pieces and using forceps. An excellent complement to avoid having to supplement the diet with vitamin supplements.
-Anchovies. For turtles and some biggie. It is important to pass over, as the head and scrapes are very important nutrients for them. I recommend giving it in a bowl apart from its housing, then very dirty.
addition, chicken meat (ideal for emergencies), mouse pups (if our scruples permitting) live food like crickets, mealworms, insects zoophobas ... never garden they may have pesticides, are a good idea as sporadic food for our turtle. The ham is not adequate: Has salt concentrations can be toxic to animals.
And remember: NO the prawns!
Friday, August 31, 2007
How To Make Apvcsausage Stuffer
Embryo-mania: Freezing mouse embryos. More on phallocentrism
(Idea for tuning Blog gun Shora )
Friends!
Today we will learn how to freeze mouse embryos, in a fast, (ultrarapid freezing), simple and for the whole family.
This system has the advantage of being faster than conventional freezing (10 minutes instead of two hours) and also avoids having to have a biocongelador. The main drawback is that it can only be used in relatively small cells, so often used to compacted morula stage, ie in embryos obtained on day 3 in the afternoon, considering day 1 on the appearance of vaginal plug, which is pregnancy diagnosis used in mice: The semen of the male has a sticky last fraction compact forming a plug in the vagina of the female, which may be visible to the morning after intercourse.
need the following materials:
Females Males
mouse
mouse HCG (human chorionic gonadotropin)
PMSG (equine chorionic gonadotropin)
Alcohol burner Paper towel
90% ethanol surgical material (normal scissors fine point, two-tooth forceps and two needle nose pliers)
binocular magnifier glass pasteur pipettes
siliconized tube system to handle the embryos into the pipette. Petri plates
Middle
M2 35mm tempered in hot plate at 37 ° C
fine hypodermic needle (no.)
Hyaluronidase. Middle
freezing. Middle
defrost Straws
Empajuelador
Bank of liquid nitrogen nitrogen insulated box Rack
liquid metal foil thickness 2mm
STEP 1: FOR EMBRYO SUPEROVULATION in morula stage animalario
-Hours: The light comes on at 7:00 and off at 21:00. This step is critical for coupling the daily hormonal rhythm of mice to which we are going to impose.
-Intraperitoneal injection of PMSG at 15:00 of the day-
-2 intraperitoneal injection of HCG at 15:00 on day 0
-are placed with males in the day 0, popping the cap 1 day ago
-sacrifice of the females on day 3 from 17:00. The embryos are at uterotubal union, so it will be necessary washed oviduct and uterine horn on each side.
STEP 2: PREPARATION OF THE MEANS OF FREEZING
"He uses the same means by which embryos are collected (usually M2). Penetrating cryoprotectants are added (low molecular weight alcohols such as glycerol or ethylene glycol) and non-penetrating (macromolecular lining the embryo and help your dehydration, such as sucrose, bovine serum albumin or fetal calf serum.
- An example would be, M2:
• 5% fetal bovine
· 0.25 M sucrose
· 3M ethylene glycol.
-is sterilized by filtration, and before use must be shaking, because the ethylene glycol has a tendency , to choose and stick to the walls of the bottle. Is kept in the refrigerator for a month, although it is better to have the shortest possible time.
STEP 3: PREPARING THE MIDDLE OF THAWING
"It's PBS with 5% fetal bovine serum, but in this case takes 0.5 M sucrose. Receive the same treatment and behaves like that of freezing.
STEP 4: METHOD OF FREEZING
few minutes before starting the procedure, place a sheet of aluminum of about 2mm in thickness in a polystyrene box filled with liquid nitrogen so that the distance between the surface of nitrogen and the plate is 50mm, and is held in a to stay fully horizontal. (With a rack or similar method)
Place a drop of about 75 ul of freezing it on a plate, and it was pipetted into the smallest possible volume 10-15 embryos. From that moment must have 5 minutes. During that time, to gently agitate the embryos. These must be incurred due to the hyperosmotic solution and float, and later (in about 3 min) fall to the bottom, at which point you can start empajuelar.
straw was loaded into a drop of medium without embryos as 15mm, 10mm then air, then drop half with embryos air again, and a half to reach the end of the straw. Open end is sealed with heated tweezers, and when they have met the 5 minutes since the embryos entered the freezing medium, place the straw in the iron on nitrogen.
Straws 2 minutes should remain on the plate, and then immersed in liquid nitrogen. From here, you can take to the bank and stay long periods of time.
STEP 5: METHOD OF THAWING:
a) Bring the thawing medium, and pour in 2 ml PBS.
b) Remove the straws from liquid nitrogen 20 seconds and let the vapors evaporate so it does not explode when heated.
c) Browse to the bathroom to 37 ยบ for another 20 seconds
d) Dry the straw, cut the seal, resting on the thawing medium, where embryos must remain at least 5 minutes, stirring gently.
e) Wash in M2
f) To plate with droplets of KSOM * whether to grow or transfer immediately whether to do the latter, it increases the success of the operation. 
(Idea for tuning Blog gun Shora )
Friends!
Today we will learn how to freeze mouse embryos, in a fast, (ultrarapid freezing), simple and for the whole family.
This system has the advantage of being faster than conventional freezing (10 minutes instead of two hours) and also avoids having to have a biocongelador. The main drawback is that it can only be used in relatively small cells, so often used to compacted morula stage, ie in embryos obtained on day 3 in the afternoon, considering day 1 on the appearance of vaginal plug, which is pregnancy diagnosis used in mice: The semen of the male has a sticky last fraction compact forming a plug in the vagina of the female, which may be visible to the morning after intercourse.
need the following materials:
Females Males
mouse
mouse HCG (human chorionic gonadotropin)
PMSG (equine chorionic gonadotropin)
Alcohol burner Paper towel
90% ethanol surgical material (normal scissors fine point, two-tooth forceps and two needle nose pliers)
binocular magnifier glass pasteur pipettes
siliconized tube system to handle the embryos into the pipette. Petri plates
Middle
M2 35mm tempered in hot plate at 37 ° C
fine hypodermic needle (no.)
Hyaluronidase. Middle
freezing. Middle
defrost Straws
Empajuelador
Bank of liquid nitrogen nitrogen insulated box Rack
liquid metal foil thickness 2mm
STEP 1: FOR EMBRYO SUPEROVULATION in morula stage animalario
-Hours: The light comes on at 7:00 and off at 21:00. This step is critical for coupling the daily hormonal rhythm of mice to which we are going to impose.
-Intraperitoneal injection of PMSG at 15:00 of the day-
-2 intraperitoneal injection of HCG at 15:00 on day 0
-are placed with males in the day 0, popping the cap 1 day ago
-sacrifice of the females on day 3 from 17:00. The embryos are at uterotubal union, so it will be necessary washed oviduct and uterine horn on each side.
STEP 2: PREPARATION OF THE MEANS OF FREEZING
"He uses the same means by which embryos are collected (usually M2). Penetrating cryoprotectants are added (low molecular weight alcohols such as glycerol or ethylene glycol) and non-penetrating (macromolecular lining the embryo and help your dehydration, such as sucrose, bovine serum albumin or fetal calf serum.
- An example would be, M2:
• 5% fetal bovine
· 0.25 M sucrose
· 3M ethylene glycol.
-is sterilized by filtration, and before use must be shaking, because the ethylene glycol has a tendency , to choose and stick to the walls of the bottle. Is kept in the refrigerator for a month, although it is better to have the shortest possible time.
STEP 3: PREPARING THE MIDDLE OF THAWING
"It's PBS with 5% fetal bovine serum, but in this case takes 0.5 M sucrose. Receive the same treatment and behaves like that of freezing.
STEP 4: METHOD OF FREEZING
few minutes before starting the procedure, place a sheet of aluminum of about 2mm in thickness in a polystyrene box filled with liquid nitrogen so that the distance between the surface of nitrogen and the plate is 50mm, and is held in a to stay fully horizontal. (With a rack or similar method)
Place a drop of about 75 ul of freezing it on a plate, and it was pipetted into the smallest possible volume 10-15 embryos. From that moment must have 5 minutes. During that time, to gently agitate the embryos. These must be incurred due to the hyperosmotic solution and float, and later (in about 3 min) fall to the bottom, at which point you can start empajuelar.
straw was loaded into a drop of medium without embryos as 15mm, 10mm then air, then drop half with embryos air again, and a half to reach the end of the straw. Open end is sealed with heated tweezers, and when they have met the 5 minutes since the embryos entered the freezing medium, place the straw in the iron on nitrogen.
Straws 2 minutes should remain on the plate, and then immersed in liquid nitrogen. From here, you can take to the bank and stay long periods of time.
STEP 5: METHOD OF THAWING:
a) Bring the thawing medium, and pour in 2 ml PBS.
b) Remove the straws from liquid nitrogen 20 seconds and let the vapors evaporate so it does not explode when heated.
c) Browse to the bathroom to 37 ยบ for another 20 seconds
d) Dry the straw, cut the seal, resting on the thawing medium, where embryos must remain at least 5 minutes, stirring gently.
e) Wash in M2
f) To plate with droplets of KSOM * whether to grow or transfer immediately whether to do the latter, it increases the success of the operation.
Sunday, August 26, 2007
Bmx Color Painting App
and biology.
little ago talking about the blunder of a professor of physiology on the definition of clitoris, and how dangerous are the findings biased. Actually, my biggest surprise came from the forum in which that statement was, as it is a fact that the inclination macho society has been projected in many areas, but is primed especially female sexuality, the pathophysiology of sociology. And 
little ago talking about the blunder of a professor of physiology on the definition of clitoris, and how dangerous are the findings biased. Actually, my biggest surprise came from the forum in which that statement was, as it is a fact that the inclination macho society has been projected in many areas, but is primed especially female sexuality, the pathophysiology of sociology. And
is no need to go back to Freud and origin "cancer" of the word hysteria ... is much closer than this: When someone tells a child that there are differences "down there", usually be something like this:
And that you have there is the tail. The girls have no tail but have a hole.
Do you find a bug in the previous discussion? Has anyone ever heard a child tell his genitals, saying he has no clitoris, but has a bag with two balls? It is equivalent.
And this is where I contradict the observers: "A penis," says this school of thought, is much more obvious to see that a clitoris, and that is why an explanation and " Vale. Well. And the teeth are much more obvious to see that the wheels, why not stop telling a child that has both.
But there's more: daddy puts
a seed in mom, and that seed grows and grows inside your tummy, even when it is ready, a baby born from it.
live the "moms-pot" enriched mitochondrial DNA!
And, even in something as seemingly trivial, has canceled all maternal contribution, aside from his role in nurturing machine. An embryo is formed from a zygote, a cell formed by half of the chromosomes of potato, and half of the chromosomes from mom. What is the difference? Well, mom is sighted, and puts their chromosomes in a single cell, full of nutrients, huge, and so protected, Dad (Which is also sighted) has to spend all their energy into making many cells and very mobile, so that at least one arrives. So far, the contribution of both is exactly the same.
But to be a dad, and single dad who "decide" the sex of the embryo (talking about mammals), as all eggs carry an X chromosome breast, while among the sperm of Dad, will you carry X and who carry Y.
only will mom and mother who shall transmit the mitochondrial DNA, which therefore propagates through the mother (grandmother, mother, daughter ...)
When egg and sperm fuse, also do their pronuclei, with your copy (each 50%) of the genetic material of the future embryo. Sexual reproduction has many advantages, such as invalid or replace damaged genes, while in other cases, the two versions of the same gene can undergo phenomena of dominance-recessive or codominance, to model the phenotype of future living.
However, in addition to energy reserves for the early stages of the embryo in the egg is also the "machinery" necessary to take advantage: the mitochondria. These remarkable organelles are not entirely dependent kernel for replication, but travel with their own genetic material and may be considered independently of the cell to which they belong, probably because of its origin (endosymbiont theory Lynn Margulis)
Since this genetic material is transmitted in full from one parent (the mother) is very suitable for tracking sources species, common ancestry and migration.
Friday, August 24, 2007
Sunday, August 19, 2007
Houses For Lease In Mckinney
anhydrobiosis is called the ability to survive on high dehydration as a result of drying atmospheric air. We observe this phenomenon
in many small aquatic animals (nematodes, rotifers beloides, tardigrades freshwater and terrestrial ...) that have the capacity to enter into a state of anhydrobiosis at any stage of the life cycle or only during certain stages of life, as with cladoraceas eggs (water fleas), the larvae of some flies, embryonic cysts coelenterates, etc.
In the case of frozen embryos of Artemia, one of the best studied cases, living desiccated persists does not mean that any water molecule. Proteins and other molecules are able to preserve what is called "structural water", ie molecules water trapped in the structures of other compounds, or are part of cohesive and adhesive bonding. This phenomenon is favored under conditions of high humidity, but still, anhydrobiotic forms contain less than 2% of water each hydrated state.
The most surprising feature of this vital state, is temporarily away signs of metabolic activity: These forms stop using O 2 , and accumulating waste products. But at any given time, under favorable conditions, the body can resume metabolism and "back to life."
In these organisms, it is often the accumulation of a disaccharide called trehalose, which prevents dehydration destabilize vital cellular structures, membranes, macromolecules ...
Animals anhydrobiosis state will tolerate many extreme conditions, as well as dehydration. And this state can be maintained for many years. Represents a highly useful evolutionary strategy, because these animals can be transported by air currents to other areas where conditions are more favorable, and once there, to establish a new colony to fall into the water.
Subscribe to:
Posts (Atom)